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. 2020 Jan 13;3:25. doi: 10.1038/s42003-020-0753-3

Fig. 5. Poly-Ub, p62, and NDP52 regulate PcLV formation interdependently.

Fig. 5

a, b FIP200 KO MEFs/GFP-LC3 or FIP200 KO MEFs/mCherry-LC3/GFP-NDP52 infected with WT or ∆ply pneumococci for 1 h with or without PYR-41 were stained with DAPI and antibodies against poly-Ub or p62, and percentages of each marker-positive bacteria containing cells were quantified. c, d FIP200 KO MEFs/mCherry-LC3 treated with indicated siRNA were infected with pneumococci for 1 h and stained with DAPI and anti-poly-Ub antibody, and percentages of LC3- or poly-Ub-positive bacteria containing cells were quantified. e, f FIP200 KO MEFs/mCherry-LC3 stably expressing GFP-p62 or GFP-NDP52 treated with indicated siRNA were infected with pneumococci for 1 h and stained with DAPI, and percentages of each marker-positive bacteria containing cells were quantified. g FIP200 KO MEFs/mCherry-LC3/GFP-NDP52 treated with indicated siRNA were infected with pneumococci for 1 h and stained with DAPI, and percentages of each marker-positive bacteria containing cells were quantified. h, i FIP200 KO MEFs/GFP-LC3 or FIP200 KO MEFs/mCherry-LC3/GFP-Lact-C2 infected with WT pneumococci for 1 or 2 h were stained with DAPI and antibodies against p62 or poly-Ub. j Autophagy machinery protein dynamics in PcLV formation. Data are expressed as mean ± SEM.; *P < 0.01.