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. 2020 Jan 13;10:156. doi: 10.1038/s41598-019-57057-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Zeta potential measurement: Surface charge was measured after sequential layering of Chitosan, Alginate and PF127 on HSCs. (b) Bar Graph representing the quantity of PF127 grafted on the HSCs after coating with PF127-CA and PF127-HSCBpep measured by Cobalt thiocyanate assay; (i) quantity in micrograms per 10,000 cells and (ii) quantity in nanograms per cell (n = 3, results as mean ± SD).(c) Size of pMVs was determined by Dynamic Light Scattering (DLS): Three peaks and polydispersity (PDi) value of 0.9 suggest that pMVs formed have very broad size distribution. (d) Bar graph representing the results of 96-well pMVs binding assay. The results suggest that out of PF127, PF68, and PEG/PEO, the higher numbers of pMVs were found attached to the well coated with PF127. (n = 3, results as mean ± SD).