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. 2020 Jan 13;10:156. doi: 10.1038/s41598-019-57057-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Flow cytometry: Bar graph representing the expression levels of CXCR4 on pMVs treated PF127-CA HSCs and HSCs. (n = 3, results as mean ± SD). (b) Bar graph representing the results of TVM assay conducted by using human bone marrow endothelial cells and PF127-CA HSCs. (n = 3, results as mean ± SD). (c) Flow cytometry: Bar graph representing the results of in vivo TVM experiments obtained revealing the percentages of PKH26 labeled PF127-CA HSCs transmigrated to the nude mice bone marrow after 24 hrs of intravenous infusion (n = 6, results as mean ± SD) (d) Flow cytometry: Bar graph representing the results of in vivo TVM experiments revealing the percentages of PKH26 labeled PF127-HSCBpep HSCs transmigrated to the nude mice bone marrow after 24 hrs of intravenous infusion (n = 6, results as mean ± SD). (e) Flow cytometry: Bar graph comparing the specificity of PF127 with other triblock copolymers (PF68) and long circulating compound (PEG/PEO) (n = 6, results as mean ± SD).