Table 1.
Analysis and computation of number of subset cells and specific cell types likely to have transmigrated to bone marrow.
| Treatment of mice | Percent total | No. of total subset cells in bone marrow | Specific cell subtypes in subset infused (in folds) | Comparison of SRC/617 and LTC-IC/103 subset cells in bone marrow in different treatments (in folds) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CD | LD | CD | LD | SRCs | LTC-ICs | ||||||
| CD | LD | ||||||||||
| A | Nil | — | — | — | — | — | — | — | — | SRCs | LTC-ICs |
| B | Only HSCs | 7.0 ± 3.6 | 6.3 ± 2.5 | 1120 | 1008 | 1.8 | 1.6 | 22.4–112 | 20–100 |
B:A(CD) = 1.8 B:A(LD) = 1.6 C:B(CD) = 7.1 C:B(LD) = 7.9 D:B(CD) = 4.4 D:B(LD) = 4.8 E:B(CD) = 2.0 E:B(LD) = 1.93 D:C(CD) = 1.6 (less) D:C(LD) = 1.64 (less) C:E(CD) = 3.45 (less) C:E(LD) = 4.09 (less) |
B:A(CD) = 22.4–112 B:A(LD) = 20–100 C:B(CD) = 6.96–6.96 C:B(LD) = 7.8–7.8 D:B(CD) = 4.3–4.37 D:B(LD) = 4.7–4.76 E:B(CD) = 1.7–17.85 E:B(LD) = 1.98–19.2 E:C(CD) = 4.0 (less) E:C(LD) = 3.9 (less) D:C(CD) = 1.61–15.9 (less) D:C(LD) = 1.65–1.63 (less) E:D(CD) = 2.45 (less) E:D(LD) = 2.45 (less) |
| C | PF127–CA HSCs | 49.3 ± 3.0 | 48.9 ± 3.05 | 7888 | 7824 | 12.78 | 12.68 | 156–780 | 156–782 | ||
| D | PF127–HSCBpep HSCs | 30.88 ± 3.7 | 29.8 ± 6.04 | 4940 | 4768 | 8.0 | 7.7 | 98–490 | 94–476 | ||
| E | CA-HSCs | 11.85 ± 0.99 | 11.98 ± 1.0 | 2022 | 1920 | 3.7 | 3.1 | 20–200 | 40–192 | ||
No. of infused cells/mouse = 16000. Cell number detected 24 hrs post infusion in bone marrow as described in text. CD: conditioning dose; LD: lethal dose; SRC: SCID-repopulating cells; LTC-IC: long-term-culture-initiating cells.