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. 2020 Jan 13;11(1):25. doi: 10.1038/s41419-019-2221-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b Quantitative real-time PCR (a) and Western blot (b) analyses of SIK2 expression in ovarian cancer A2780 and SKOV3 cells with treatment as indicated. siSIK2–1 and siSIK2–2, siRNAs against SIK2; siCtrl, control siRNA; SIK2, expression vector encoding SIK2; EV, empty vector. c–f Cellular content of free fatty acid (c), triglyceride (d), phospholipids (e) and cholesterol (f) were detected in A2780 and SKOV3 cells with treatment as indicated. g The content of neutral lipids were detected by BODIPY 493/503 dye and counterstained with DAPI in A2780 and SKOV3 cells with treatment as indicated. Scale bars, 50 μm. Average numbers of LDs per cell (upper panel), percentage of cellular area occupied by LDs (lower panel). Data are shown as mean ± S.E.M from three independent experiments. *p < 0.05; **p < 0.01.