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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Microbiol Methods. 2019 Oct 31;167:105760. doi: 10.1016/j.mimet.2019.105760

Figure 4.

Figure 4.

Experimental Controls including Detergent Treated Cells assayed by Flow Cytometry. Bacteria were cultured by shaking to generate planktonic cells (3h).

Bacteria were stained with SYTO 60 and TOTO-1 and processed for flow cytometry. The top panels feature specificity controls, from L to R: unstained, SYTO 60 only, and TOTO-1 only. The bottom panels: Triton-X 100 pretreated (30 min, 10% Triton-X 100 prior to staining) planktonic culture WT PA01 (L); and planktonic culture PA14 (R). All samples were gated on SSC/FSC and threshold level set to SSC 200. Representative images for three independent experiments are shown.