Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 13;10:172. doi: 10.1038/s41598-019-55909-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of DOX loading in CELVEC performance. (A) Flow cytometry dot plot depicting PS on CELVEC (blue) and DOX@CELVEC (green) surface 4 h post-generation. (B) Quantitative assessment of flow cytometry dot plot data exhibiting DOX and Annexin V positive cells using gates established in (A). (C) Cytotoxic evaluation of murine macrophage cells following electroporation (CELVEC, blue) and electroporation supplemented with DOX (DOX@CELVEC, green) to create DOX@CELVEC. Neutral red uptake was employed to evaluate cellular viability and proliferation over 72 h. Doxorubicin loading was performed at 20 mg/mL. Values normalized to untreated murine macrophages (CTRL). (D) Quantification of CELVEC adhered to TNF-α-activated HUVEC following 30 min of in vivo-like flow conditions of 0.1 dyn/cm². (E) Representative fluorescent microscope images used for (D,E) depicting CELVEC adhesion following 30 min. of flow. Scale bar, 100 μm. The data is plotted as the mean ± SEM. ***p < 0.001.