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. 2020 Jan 13;10:191. doi: 10.1038/s41598-019-57051-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genetic Modification of PCC 11802. (A) Construction of modified vector by replacing the neutral site I (NSI) homology arms of PCC 7942 in the pSyn_1 plasmid with those of PCC 11801 by PIPE cloning method. (B) Microscopic images of wildtype and recombinant cells of PCC 11802 showing chlorophyll a fluorescence (left panels) and eYFP fluorescence (right panels), and (C) The activity of phosphoenolpyruvate carboxylase (PEPC) protein in wildtype and recombinant cells overexpressing PEPC protein grown under 1% CO₂ in shake flasks. The activity data is plotted on log₁₀ scale.