Table 2.
The function-based comparison between Synechococcus elongatus PCC 11802 and 11801 identified using RAST tool. The genes annotated only in PCC 11802 compared to PCC 11801 genome and vice-versa are represented. The obtained results were also verified by comparing the obtained protein sequence against the whole genome sequence using the NCBI BLAST.
| Locus Tag† | Protein ID† | Putative Role | Remarks based on previous reports | |
|---|---|---|---|---|
| Proteins unique to PCC 11802 | ||||
| Type III restriction-modification system methylation subunit/site-specific DNA- methyltransferase | 1823836–1821905* | peg.1952# | DNA methylation | May be involved in epigenetic regulation of genes through differential methylation of genes77,78 |
| Retron-type RNA-directed DNA polymerase | EKO22_06085 | QFZ92002 | Reverse transcription | Uses RNA as a template as well as the primer for reverse transcription79,80 |
| Programmed cell death antitoxin MazE like | EKO22_08965 | QFZ92458 | Programmed cell death/Reversible Growth Arrest | Toxin-antitoxin system (TAS) responsible for heat-induced programmed cell death in Synechocystis sp. PCC 680381 |
| Programmed cell death toxin MazF like | EKO22_08970 | QFZ92459 | ||
| RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE) | EKO22_03050 | QFZ91496 | Involved in the adjustment of nutrient consumption under starvation conditions44–46 | |
| VapB protein (antitoxin to VapC) | EKO22_08890 | QFZ92444 | ||
| VapC toxin protein | EKO22_08885 | QFZ92443 | ||
| YefM protein (antitoxin to YoeB) | EKO22_07885 | QFZ92286 | Survival of cells in prolonged exposure to stress | This TAS system has been shown to be involved in cell survival under prolonged exposure to antibiotics by entering a dormant state82 |
| YoeB toxin protein | EKO22_07880 | QFZ92285 | ||
| Proteins unique to PCC 11801 | ||||
| Putative ski2-type helicase MJ1124 | DOP62_06670 | AZB72445 | RNA degradation, processing, and splicing | Demonstrated to activate RNA degradation in eukaryotes83 |
| HigA protein (antitoxin to HigB) | DOP62_07875 | AZB72637 | Reversible Growth Arrest | These are found to be overexpressed in Mycobacterium tuberculosis under different stress conditions such as heat shock, nutrient starvation, DNA damage and hypoxia84 |
| HigB toxin protein | DOP62_07880 | AZB72638 | ||
†The IDs are based on annotations obtained through NCBI Prokaryotic Genome Annotation Pipeline.
*The coordinates of gene annotated as a single protein by RAST have been provided instead of NCBI locus tag since a translated protein sequence was not obtained using NCBI Prokaryotic Genome Annotation Pipeline.
#RAST protein ID is provided instead of NCBI protein ID. The protein sequence can be obtained from Supplementary File S-1 (Table S3).