Figure 6.
Yap supresses Gdf5 expression. (A,B) C3H10T1/2 cells were transduced with retrovirus encoding YAP1 or constitutively active YAP1S127A, or with empty vector (Control), and cultured in standard monolayer (n = 5 experiments), or in high-density micromass for 1 day (n = 4 experiments; data from separate experiments). (C–F) C3H10T1/2 cells were transfected with DsiRNA to knock down Yap, or Yap and Taz, or with mismatch DsiRNA (Control), and cultured for 4 days in chondrogenic micromass culture. Gdf5 (A,E), Wnt9A (B,F), Yap (C) and Taz (D) expression were determined by quantitative RT-PCR. All data were normalised to expression of Hprt1, and are shown relative to expression of the gene of interest in non-transduced cells in monolayer (A,B), or in micromass (C–F). *p < 0.05; **p < 0.01; ***p < 0.001, based on two-way ANOVA with Holm-Sidak post-test for pairwise comparisons (A,B) or one-way ANOVA with Holm-Sidak post-test for comparisons against control (C–E).