Table 2.
Primary antibodies used for double labeling with ECM molecules in the rat
| Anti-neurofilament | Anti-neurofilament | Anti-MAP2a | |
|---|---|---|---|
| Supplier, cat. no | Sigma-Aldrich; N4142 | Sigma-Aldrich; N5389 | Merck Millipore; AB5622 |
| Species of origin, type | Rabbit; polyclonal, IgG | Mouse; monoclonal, IgG1 | Rabbit; polyclonal, IgG |
| Immunogen | Purified neurofilament 200 from bovine spinal cord | Purified neurofilament 200 from pig spinal cord | Purified Microtubule-associated protein from rat brain |
| Dilution | 1:80 | 1:40 | 1:500 |
aMicrotubule-Associated Protein 2