. 2019 Nov 28;225(1):129–148. doi: 10.1007/s00429-019-01991-w

Table 2.

Summarised description of antibodies used

Antigen	Immunogen	Description	Dilution used
GluA2/3	Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI)	Polyclonal Rabbit, Merck Millipore #AB1506; RRID:AB_90710	1:100 TBS 0.05 M + Triton-Tx 0.3%
GAD67	Recombinant GAD67 protein	Monoclonal mouse, Merck Millipore #MAB5406 clone 1G10.2 RRID:AB_2278725	1:1000 TBS 0.05 M + Triton-Tx 0.3%
Parvalbumin	Rat muscle parvalbumin	Polyclonal Rabbit, Swant #PV-25; RRID:AB_10000344	1:5000 TBS 0.05 M + Triton-Tx 0.3%

Antigen

Immunogen

Description

Dilution used

GluA2/3

Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI)

Polyclonal Rabbit, Merck Millipore #AB1506; RRID:AB_90710

1:100

TBS 0.05 M + Triton-Tx 0.3%

GAD67

Recombinant GAD67 protein

Monoclonal mouse, Merck Millipore #MAB5406 clone 1G10.2 RRID:AB_2278725

1:1000

TBS 0.05 M + Triton-Tx 0.3%

Parvalbumin

Rat muscle parvalbumin

Polyclonal Rabbit, Swant #PV-25; RRID:AB_10000344

1:5000

TBS 0.05 M + Triton-Tx 0.3%

Group 3: GluA2/3 AMPA R subunits immunocytochemistry. Control group n: 4; bilateral deaf animals n: 4

Group 4: GAD67 and PV immunocytochemistry. Control group n: 3; bilateral deaf animals n: 5