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. 2020 Jan 13;11:239. doi: 10.1038/s41467-019-13897-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b A confocal image series (a) and c, d a STED image series (c) of live HeLa cells transfected with emiRFP703-vimentin. The graphs (b, d) show the normalized integrated intensity of the recorded frames (red circles) for both image series, plotted every 40th frame for the confocal for better visualization (b) and every frame for STED (d). The displayed intensity scale has been adjusted per frame from zero to the maximum in respective frame. Scale bars, 2 µm.