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. Author manuscript; available in PMC: 2021 Feb 5.
Published in final edited form as: Biochem Biophys Res Commun. 2019 Nov 21;522(2):415–421. doi: 10.1016/j.bbrc.2019.11.110

Fig. 2. Calcium influx triggers ESCRT-III accumulation in plasma membrane during ferroptosis.

Fig. 2.

(A) Western blot analysis of EIF2AK3 and HSPA5 expression in whole cell extracts in PANC1 cells following treatment with erastin (10 μM) or RSL3 (0.5 μM) for 12 h. (B) Analysis of cytosolic calcium level in PANC1 cells following treatment with erastin (10 μM) or RSL3 (0.5 μM) for 12 h (n = 3, *P < 0.05). (C) Western blot analysis of CHMP5, CHMP6, and SLC7A11 expression in isolated plasma membrane in PANC1 cells following treatment with erastin (10 μM) or RSL3 (0.5 μM) in the absence or presence of PD98059 (10 μM), TUDCA (50 μM), or BAPTA-AM (10 μM) for 12 h.