Fig. 3. Inhibition of ESCRT-III promotes ferroptosis.
(A) Western blot analysis of CHMP5 and CHMP6 expression in indicated gene knockdown PANC1 or HepG2 cells. (B-E) Analysis of cell death (B), intracellular MDA (C), intracellular iron (D), and HMGB1 release (E) in indicated PANC1 or HepG2 cells in response to erastin (10 μM) or RSL3 (0.5 μM) for 12 h (n = 3, *P < 0.05).
