Fig. 4. Effects of genetic inhibition of CHMP5 and CHMP6 on ferroptosis in vivo.
(A) Athymic nude mice were injected subcutaneously with the indicated PANC1 or HepG2 cells for 7 days and then treated with RSL3 (30 mg/kg; i.p., once every other day) at day 7 for 2 weeks. Tumor volumes were calculated weekly (n = 5 mice/group, *P < 0.05). (B-C) In parallel, MDA levels (B) in isolated tumors or serum HMGB1 at day 14 after treatment were assayed (n = 3 mice/group, *P < 0.05).
