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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Exp Eye Res. 2019 Oct 31;190:107864. doi: 10.1016/j.exer.2019.107864

Fig. 4. MalK- and PropK-modified proteins in human lens epithelial cells.

Fig. 4.

FHL124 cell lysates were subjected to western blotting. Membranes were incubated with the primary antibody for MalK or PropK (A and C) or with the primary antibody pre-incubated with malonylated or propionylated lysozyme (B and D). Densitometric plots for proteins within open brackets, normalized to β-actin (loading control) are shown on the right. **p<0.005. n=4. Subcellular fractions of FHL124 cells were analyzed for MalK (E) or PropK (F)-modified proteins by western blotting. To confirm fractionation, the cytosolic, mitochondrial and nuclear fractions were probed in western blots for GAPDH (G), SOD2 (H) and histone H3 (I), respectively. c, cytosolic; m, mitochondrial; n, nuclear. M, molecular weight markers.