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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Exp Eye Res. 2019 Oct 31;190:107864. doi: 10.1016/j.exer.2019.107864

Fig. 6. SIRT3 and SIRT5 are enzymatically active in cultured human lens epithelial cells but not in human lens homogenates.

Fig. 6.

Deacylation of propionylated or malonylated lysozyme by the lens epithelial cell lysate (A and B) and WS human lens proteins (C and D) was determined by western blotting using an antibody against PropK or MalK. Deacylation of acylated lysozyme by the mouse heart extract was used as the positive control (lane 5 in all panels). Densitometric plots for the acylation levels in lysozyme are also shown. The bar graphs represent the means ± standard deviation of triplicate measurements. The Ponceau stained membrane images of respective blots are also shown to demonstrate equal protein loading. M, molecular weight markers; lane 1, acylated lysozyme alone; lane 2, WS human lens protein; lane 3, acylated lysozyme + WS human lens protein; lane 4, WS mouse heart protein; lane 5, acylated lysozyme + WS mouse heart protein. NS, non-significant; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.