. Author manuscript; available in PMC: 2021 Jan 1.

Published in final edited form as: Exp Eye Res. 2019 Oct 31;190:107864. doi: 10.1016/j.exer.2019.107864

Table 1.

MalK and PropK modification sites in α-crystallins.

		Malonylation		Propionylation
Protein	Mod Site	Age 26	Age 71	Age 26	Age 71
αA-crystallin	K70	-	-	Flagged	ms/ms
	K99	-	-	ms/ms	ms/ms
	K166	ms/ms	ms/ms	ms/ms	EIC
αB-crystallin	K72	-	-	Flagged	ms/ms
	K90	ms/ms	ms/ms	ms/ms	ms/ms
	K92	ms/ms	ms/ms	EIC	ms/ms
	K103	-	-	ms/ms	ms/ms
	K166	ms/ms	ms/ms	ms/ms	ms/ms
	K174	-	-	EIC	ms/ms

Mod Site text Black – Lysine site found to be both malonylated and propionylated

Mod Site text Blue – Lysine site found to be only malonylated

Mod Site text Red – Lysine site found to be only propionylated

ms/ms – quality fragmentation spectrum match that passed quality Peptide Score, SPI%, and VΜL score cut-off filters

EIC – Extracted Ion Chromatogram (EIC) peak present based on the retention time (less than 0.15 min shift) and mass (less than 5ppm) of the peptide matched during database searching in the other aged lens sample

Flagged –No EIC peak present based on the retention time (less than 0.15 min shift) and mass (less than 5ppm) of the peptide matched during database searching in the other aged lens sample