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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: FEBS Lett. 2019 Aug 31;594(1):144–152. doi: 10.1002/1873-3468.13581

Figure 5.

Figure 5.

The P2X5 C-terminal region is required for osteoclast maturation. (A) WT and P2rx5−/− BMMs were retrovirally transduced with the indicated vectors. Overexpression of transduced genes (mP2×5 and mP2×5Δ268) was confirmed by western blot. (B) WT and P2X5−/− BMMs retrovirally transduced with the indicated vectors were cultured with M-CSF + RANKL for three days to induce osteoclasts. TRAP activity, frequency of TRAP+ multinucleated cells (3 nuclei or more per cell), and frequency TRAP+ hyper-multinucleated cells (>100 μm) are shown. Data are shown as mean ± S.D. **; p<0.01, ***; p<0.001, NS; not significant.