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. Author manuscript; available in PMC: 2021 Jan 15.
Published in final edited form as: Virology. 2019 Nov 15;540:119–131. doi: 10.1016/j.virol.2019.11.010

Figure 3. siRNA-mediated GADD34 knockdown induces HIV-1 gene expression.

Figure 3.

A-B) MT-2 and Jurkat cells were transfected by electroporation with 1.0 uM siRNA as indicated in the presence of 5.0 μg of pNL4-3.Luc.R-E- plasmid for 36 h. Cells were harvested for Western blot analysis (A) with GADD34 specific antibodies (MT2 cells) or RNA isolation for RT-PCR (B) with GADD34 and GAPDH specific primers (Jurkat cells). C-D) Cell lysates from transfected MT-2 (C) and Jurkat (D) were processed for luciferase measurement.