Table 1. Summary of parameters for PilB and ligand binding with or without effectors.
| PilBN | PilBΔN | Full-length PilB | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ligand1 | cdG | ATPγS | ADP | cdG | ATPγS | ADP | cdG | cdG | ATPγS | ADP |
| Effector2 | None | None | None | None | None | None | ATPγS | ADP | cdG | cdG |
| KD3 | 107±14* | 5.2±0.7 | 7.3±0.5 | 53±10* | 3.8±0.5 | 4.3±1.0 | 58±15* | 18±6* | 4.0±0.5 | 9.0±0.7 |
| ΔG | −9.5 | −7.2 | −7.0 | −9.9 | −7.4 | −7.3 | −9.9 | −10.6 | −7.4 | −6.9 |
| ΔH | −7.4±0.5 | −11.5±0.6 | −16.2±0.4 | −14.5±0.13 | −10.3±0.3 | −15.4±1.0 | −14.0±0.3 | −12.0±0.09 | −10.3±0.3 | −46.2±1.6 |
| −TΔS | −2.1 | 4.3 | 9.2 | 4.6 | 2.9 | 8.1 | 4.1 | 1.5 | 2.9 | 39.4 |
| N | 1.09±0.03 | 2.42±0.07 | 2.20±0.03 | 5.83±0.01 | 2.8±0.06 | 2.20±0.02 | 6.33±0.08 | 5.94±0.09 | 2.91±0.04 | 1.79±0.06 |
1
Ligand represents the compound that was titrated into the sample cell with the specified protein;
2
Effector refers the chemical that was pre-incubated with the protein;
3
The apparent dissociation constant (KD) is in micromolar (μm) except those for cdG (*) which is in nanomolar (nM). ΔG, ΔH, and TΔS are in kilocalorie per mole (kcal/mol). N is the number of ligand binding site per protein.