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. 2020 Jan 7;9:e00135. doi: 10.1016/j.parepi.2020.e00135

Fig. 2.

Fig. 2

Example of an agarose gel presenting the DNA profiles resulting from the digestion of amplified DNA fragments of the TbNT10 gene of T. congolense with DpnII. Lane 1: internal positive controls made up of a DNA mixture of resistant and sensitive T. congolense strains; lanes 2, 5, 6, 7 and 8: DNA samples from diminazene aceturate sensitive strains of T. congolense (one DNA fragment illustrating non digestion of the TbNT10 gene by DpnII); lanes 3 and 4: DNA samples from diminazene aceturate resistant strains of T. congolense (two DNA fragments after the digestion with DpnII); lane 9: negative control (nuclease-free water); MW marker: 100 bp DNA ladder (New England Biolabs).