Table 3.
Cell Surface and Intracellular Staining Panel 2
| Marker | Dye | Host species | Reactivity | Clone | Description | Catalog no |
|---|---|---|---|---|---|---|
| CD3 | Alexa Fluor 700 | Mouse | Human | UCHT1 | Co-receptor: CD3 T cells | 300424 |
| CD4 | PE | Mouse | Human | RPA-T4 | Co-receptor: CD4 T cells | 561843 |
| CD8 | BUV 496 | Mouse | Human | RPA-T8 | Co-receptor: CD8 T cells | 564804 |
| CD19 | PE/Cy5 | Mouse | Human | J3-119 | Co-receptor: B cells | A07771 |
| CD14 | PE/Cy5 | Mouse | Human | RMO52 | Co-receptor: monocytes | A07765 |
| CD137 | PE/Dazzle | Mouse | Human | 4B4-1 | Costimulatory receptor | 309826 |
| CD278 (ICOS) | Brilliant Violet 510 | Mouse | Human | C398.4A | Costimulatory receptor | 313525 |
| CD38 | Brilliant Violet 650 | Mouse | Human | HB-7 | cADP ribose hydrolase | 356620 |
| TIGIT (VSTM3) | PerCP-eFluor 710 | Mouse | Human | MBSA43 | Inhibitory receptor | 46-9500-42 |
| PD1 | Brilliant Violet 785 | Mouse | Human | EH12.2H7 | Inhibitory receptor | 329930 |
| IL22 | PE | Mouse | Human | 2G12A41 | Proinflammatory cytokine | 366704 |
| TNF-α | PE/Cy7 | Mouse | Human | MAb11 | Proinflammatory cytokine | 502930 |
| IFN-γ | APC/Cy7 | Mouse | Human | 4S.B3 | Proinflammatory cytokine | 502530 |
| Granzyme B | FITC | Mouse | Human/mouse | GB11 | Cytotoxic molecule | 515403 |
| Perforin | Brilliant Violet 421 | Mouse | Human | B-D48 | Cytotoxic molecule | 353307 |
cADP, cyclic adenosine diphosphate; DAPI, 4′,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; PE, phycoerythrin.