Table 4.
Cell Surface and Intracellular Staining Panel 4
| Marker | Dye | Host species | Reactivity | Clone | Description | Catalog no |
|---|---|---|---|---|---|---|
| CD3 | Alexa Fluor 700 | Mouse | Human | UCHT1 | Co-receptor: CD3 T cells | 300424 |
| CD4 | Brilliant Violet 711 | Mouse | Human | OKT4 | Co-receptor: CD4 T cells | 317440 |
| CD8 | BUV 496 | Mouse | Human | RPA-T8 | Co-receptor: CD8 T cells | 564804 |
| CD19 | PE/Cy5 | Mouse | Human | J3-119 | Co-receptor: B cells | A07771 |
| CD14 | PE/Cy5 | Mouse | Human | RMO52 | Co-receptor: monocytes | A07765 |
| CD137 | PE/Dazzle 594 | Mouse | Human | 4B4-1 | Costimulatory receptor | 309826 |
| CD278 (ICOS) | Brilliant Violet 510 | Mouse | Human | C398.4A | Inhibitory receptor | 313525 |
| CD38 | Brilliant Violet 650 | Mouse | Human | HB-7 | cADP ribose hydrolase | 356620 |
| PD1 | Brilliant Violet 785 | Mouse | Human | EH12.2H7 | Inhibitory receptor | 329930 |
| IFN-γ | APC/Cy7 | Mouse | Human | 4S.B3 | Proinflammatory cytokine | 502530 |
| IL13 | PE/Cy7 | Mouse | Human | JES10-5A2 | Proinflammatory cytokine | 501914 |
| Granzyme B | FITC | Mouse | Human/mouse | GB11 | Cytotoxic molecule | 515403 |
| T-bet | PE | Mouse | Human | 4B10 | Transcription factor–Th1 polarized T cell | 137236 |
| GATA-3 | Brilliant Violet 711 | Mouse | Human | 16E10A23 | Transcription factor–Th2 polarized T cell | 653814 |
| RORγt | APC | Mouse | Human/mouse | AFKJS-9 | Transcription factor–Th17 polarized T cell | 17-6988-80 |
cADP, cyclic adenosine diphosphate; FITC, fluorescein isothiocyanate; PE, phycoerythrin.