Fig. 9.

(a,b) Plots of apparent rate constants (top) and signal loss at 1 h (bottom) vs. residue number obtained from the reaction of apo-SOD1^SH (a) and E,Zn-SOD1^SH (b) with 1 mM MG. Apparent rate constants were obtained from pseudo-first order mono-exponential fittings. In b, red dots indicate residues with k = 0. (c,d) Cartoon representation of the 3D structure of a SOD1 monomer (PDB 2AF2), colour-coded according to the apparent rate constants of apo-SOD1^SH (c) and E,Zn-SOD1^SH (d) from white (k = 0) to dark red (k > 0.4 h^-1). Unassigned or non-fitted residues are shown in cyan. The viewpoints with respect to the monomer (apo-SOD1^SH) or the homodimer (E,Zn-SOD1^SH) are shown at the bottom. Residues close to red spots are labelled in c; the homodimer interface and β-strands 2 and 3 are labelled in d. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)