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. 2019 Oct 5;9(2):313–333. doi: 10.1016/j.jcmgh.2019.09.009

Figure 1.

Figure 1

Characterization of changes in tissue leukocytes in response to diet-induced obesity. Four-week-old male C57BL/6J mice were purchased from The Jackson Laboratory and housed for 2 weeks to favor microbiota stabilization. Subsequently, half of the mice were switched to a Western-style, high-fat diet (60% kcal from fat) or continued on a standard grain-based chow as a control. After 8 weeks on a WSD, mice were killed and biometric measurements are shown for (A) final body weight and (B) epididymal adipose weight. (C) In addition, a 15-hour fasting blood glucose level was measured before euthanasia. Epididymal adipose tissue was analyzed by flow cytometry to quantify the following: (D) macrophages, (E) M1:M2 macrophage ratio, (F) DCs, (G) CD11b+CD103- and CD11b-CD103+ DC subsets, and (H) eosinophils and neutrophils. Colon tissue was used for flow cytometry to quantify as cells per organ: (I) DCs, (J) CD103+/- dendritic subsets, and (K) eosinophils and neutrophils. Data are the means ± SD (N=5). Statistical significance was determined using the t test. P < .05, brackets indicate significance. eAT, epididymal adipose tissue.