Figure 10.

Transplant of microbiota from obese mice mildly recapitulates features of obesity and myeloid cell alterations. Four-week-old male C57BL/6 GF mice were purchased from Taconic Biosciences and were administered feces from either chow-(blue boxes) or WSD-(orange circles)fed mice by oral gavage. These animals subsequently were maintained in a sterile environment for 4 weeks before terminal tissue collection and biometric quantification, data are as follows: (A) final body weight; (B) epididymal adipose tissue weight; (C) subcutaneous adipose tissue weight; (D) brown adipose tissue weight; (E) 15-hour fasting blood glucose level; (F) ALT concentration, AST concentration, and total serum cholesterol; (G) liver weight; (H) colon weight/length ratio; and (I) spleen weight. Epididymal adipose tissue was analyzed using flow cytometry to quantify the following: (J) macrophages, (K and L) M1 macrophages, (M and N) M2 macrophages, (O) M1:M2 ratio, and (P) eosinophils. (Q) Epididymal adipose tissue collected at death also was analyzed for cytokines via quantitative reverse-transcription polymerase chain reaction. Colon tissue was analyzed using flow cytometry to quantify the following: (R) DCs, (S) CD103^- DCs, (T) CD103⁺ DCs, and (U) neutrophils. Data are the means ± SD (N=3-4). Statistical significance was determined using the t test. P < .05, brackets indicates significance. eAT, epididymal adipose tissue; FMT, fecal microbiota transplant; mRNA, messenger RNA.