An obesogenic diet alters myeloid cell populations in the small intestine, and to a lesser extent in the spleen and PPs. (A) After 8 weeks on chow (orange circles) or a WSD (blue squares), mice (N=5) were killed and colon tissue was analyzed for macrophages. Small intestinal tissue was analyzed for the following: (B) DCs, (C) CD103+/- DCs, (D) eosinophils and neutrophils, and (E) macrophages. Splenic tissue was analyzed for (F) eosinophils, neutrophils, inflammatory monocytes, and (G) CD8+/- classical dendritic cells (cDCs). (H–I) PPs were analyzed for total DCs and DC subsets. Data are the means ± SD. Statistical significance was determined using the t test. P < .05, brackets indicate significance.