Table 1.
Staining Cocktails
| Marker | Fluorophore | Dilution | Supplier |
|---|---|---|---|
| Macrophages | |||
| CD11c | PacBlue | 1:100 | eBioscience (San Diego, CA) |
| CD301 | APC | 1:50 | Biolegend (San Diego, CA) |
| CD45 | PerCP | 1:100 | Biolegend |
| CD64 | PE/Dazzle | 1:100 | Biolegend |
| F4/80 (or CD11b) | PE | 1:100 | Biolegend |
| MHCII | AF700 | 1:100 | eBioscience |
| Dendritic cells | |||
| CD103 | APC | 1:50 | Biolegend |
| CD11b | PE | 1:100 | Biolegend |
| CD11c | PacBlue | 1:100 | Biolegend |
| CD45 | PerCP | 1:100 | Biolegend |
| CD64 | PE/Dazzle | 1:100 | Biolegend |
| MHCII | AF700 | 1:100 | eBioscience |
| Eosinophils and neutrophils | |||
| CD11b | PE | 1:100 | Biolegend |
| Ly6-G | FITC | 1:100 | Biolegend |
| MHCII | AF700 | 1:100 | eBioscience |
| Siglec-F | BV421 | 1:100 | BD Biosciences |
| CD45 | PerCP | 1:100 | Biolegend |
| Splenic tissue | |||
| CD11b | Pe/Cy5 | 1:100 | Biolegend |
| CD11c | Pe/Cy7 | 1:300 | Biolegend |
| CD19 | APC | 1:100 | eBioscience |
| CD3 | PE | 1:100 | Biolegend |
| CD45 | PerCP | 1:100 | Biolegend |
| CD8 | eFluor 450 | 1:100 | eBioscience |
| Gr-1 | FITC | 1:100 | Biolegend |
| Ly6-C | BV605 | 1:100 | Biolegend |
| Peyer's patch | |||
| CD11b | Pe/Cy5 | 1:100 | Biolegend |
| CD11c | APC | 1:100 | Biolegend |
| CD8a | eFluor 450 | 1:100 | eBioscience |
| MHCII | AF700 | 1:100 | eBioscience |