Figure 3.

Expression and subcellular localizations of H pylori–induced changes in LATS2 and YAP1 expressions in cultured gastric and nongastric cells. Time course analyses were performed in gastric AGS and MKN74 and in nongastric noncancerous epithelial cells (HMLE and RPE1) infected with the H pylori 7.13 WT strain and the cagA-deleted isogenic mutant (ΔcagA) strain for different time periods during 24 hours. (A) LATS2, LATS2-P^Thr1041, total YAP1, and YAP-P^Ser127 protein levels were analyzed by Western blot in noninfected cells, at the indicated time periods during 24 hours of HPI with WT H pylori and at 24 hours of HPI with the cagA-deleted isogenic mutant. A representative blot of 3 or more experiments is shown. The number under each band corresponds to the value of the relative protein quantification normalized to the housekeeping protein (glyceraldehyde-3-phosphate dehydrogenase [GAPDH] or α-tubulin). *P < .05 vs noninfected AGS or MKN74 cells. (B) Graphic representation of the ratio of YAP-P^Ser127 on total YAP1 during kinetics of infection of H pylori 7.13 WT in the 4 cell lines. (C and D) Representative images of the expression and subcellular localization of LATS2 and YAP1 (in green) evaluated by immunofluorescence in (C) AGS and MKN74 gastric epithelial cells at 2 and 24 hours of HPI, and (D) in RPE1 and HMLE nongastric noncancerous epithelial cell lines at 2, 5, and 24 hours of HPI. Actin was marked with phalloidin–Alexa Fluor–546 (in red) and nuclei were marked with 4’-6-diamino-phenyl-indol (in blue). Scale bars: 10 μm.