Fig. 1.

Differentiation of SVF cells isolated from WAT of female and male mice with adipocyte-specific ablation of all isoforms of Nrf1. Animals were 16 weeks of age. Groups of n = 3 mice were used. (A and F) Relative mRNA expression of Nrf1 in adipocyte fractions and SVF of gonadal WAT (gWAT) of female mice (A) and inguinal WAT (iWAT) of male mice (F). Adi-Flox, Adi-KO, SVF-Flox and SVF-KO represent adipocyte fractions and SVF of Nrf1-Flox (Flox) and Nrf1(f)-KO (KO) mice, respectively. *p < 0.05 vs the same cell type of Flox, ^#p < 0.05 vs adipocytes with the same genotype. (B) NRF1 protein expression in adipocytes of gWAT of female Flox and KO mice. Adipocytes were cultured in normal growth medium with or without 5 μM sodium arsenite, a potent NRF1 inducer, for 6 h to increase expression of NRF1. (C) Basal mRNA expression of adipogenic factors in cultured SVF cells isolated from gWAT of female mice. The cells were maintained in growth medium without hormonal additives for 5 days post confluence. (D) Representative images (10 X) of ORO staining of the cells in (C). (E) Quantification of ORO staining in Fig. 1D. (G and H) mRNA expression of adipogenesis-related factors (G) and representative images (10 X) of ORO staining (H) of differentiated SVF cells isolated from iWAT of male mice. Cultured SVF cells were differentiated using DMIRI protocol (see Methods). *p < 0.05 vs Flox. (I) Quantification of ORO staining in Fig. 1H.