Fig. 5.

Effects of overexpression of various isoforms of NRF1 on adipogenesis in 3T3-L1 cells. (A) The mRNA expression of Nrf1, Nrf2, Gclc, Gclm, Nqo1 and Ho1 in various Nrf1-OE cells. Cont, Control; 313, 453, 572, 583 and 741 refer to the cells overexpressing indicated isoforms of Nrf1, respectively. n = 3. *p < 0.05 vs. Cont. (B) Representative image of protein expression of various isoforms of NRF1 in Nrf1-OE cells. (C) Oil-red O staining of Control and Nrf1-OE cells treated with DMI protocol for 5 days. (D) The protein expression of adipogenic factors in Nrf1-OE cells treated with DMI for indicated days. Cont, Control; 741, cells overexpressing NRF1-741; D, Day. A representative result of 3-5 independent experiments is shown. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)