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. 2020 Jan 3;30:101423. doi: 10.1016/j.redox.2019.101423

Fig. 2.

Fig. 2

S-glutathionylation, DNA methylation, and histone deacetylation inhibitors did not sensitize ‘resistant’ cell lines to cold physical plasma. (A) Global protein s-glutathionylation (PSSG) in tumor cell lysates. (B) Metabolic activity of ‘resistant’ cell lines upon pretreatment with GSTP1 inhibitor ezatiostat (EZA; 25 μM). (C) Metabolic activity of ‘resistant’ cell lines upon pretreatment with DNA methylation inhibitor azacytidine (AZA; 5 μM). (D) Metabolic activity of ‘resistant’ cell lines upon pretreatment HDAC inhibitor vorinostat (VOR; 10 μM). P30s and P60s are 30s and 60s of plasma treatment time, respectively. Data are mean ± SEM from three independent experiments. Statistical analysis was performed using Student's t-test with Welch's correction (B–D). ∗∗ = p < 0.01; ∗∗∗ = p < 0.001; ns = not significant.