Fig. 6.

xCT was differentially expressed in human melanoma patients (A) Representative images of immunostaining staining of malignant metastatic melanoma samples from five patients using antibodies against melanoma tumor antigen S100 (Red) and xCT (yellow). DAPI (blue) served as the nuclear counterstain. (B) Quantification of xCT expression from (A) expressed as % xCT/S100 double-positive cells. Statistical analysis was performed by one way anova, ***p < 0.001. (C) Immunostaining of cleaved caspase 3 (red) following ex vivo treatment of human melanoma punch biopsies with cold physical plasma for 60s (P60s). DAPI (blue) served as the nuclear counterstain. (D) Quantification of cleaved caspase 3 intensity from (C) expressed as mean fluorescent intensity from 1000 DAPI positive cells. Statistical analysis was performed by student's t-test with Welch's correction, *p < 0.05. Scale bar: 250 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)