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. 2019 Oct 10;9(2):295–312. doi: 10.1016/j.jcmgh.2019.10.002

Figure 6.

Figure 6

RIPK1 kinase activity is required for TNF-induced enteroid death. (A) Ikkβ(EE)IEC enteroids were incubated with TNF (40 ng/mL) in the absence or presence of RIPK1 or RIPK3 inhibitors and photographed 4 hours later. Original magnification ×200. (B) Lysates of Ikkβ(EE)IEC enteroids treated as above were subjected to IB analysis after 4 hours. (C) WT enteroids were treated for 24 hours with interleukin-22 (10 ng/mL) in the absence or presence of Nec-1. Two hours before fixation, EdU was added to the culture medium. ****P ≤ .0001. (D) Percentages of EdU positive cells per crypt are shown. IL, interleukin; ns, not significant.