Claudin-2 expression is limited to the base colon of crypt in NKCC1WT/DFXand NKCC1DFX/DFXmice. Representative IF images of NKCC1WT/WT, NKCC1WT/DFX, and NKCC1DFX/DFX mouse colon sections stained with (A–C) claudin-2 and (D–F) claudin-1 antibodies (red). Sections also show DAPI (blue) or nuclei staining. (D–F) In contrast, claudin-1 expression at the lateral membrane was similar in all 3 genotypes. Scale bars: 20 μm. Number of claudin-2–positive cells per crypt were counted on 5 fields, 11 crypts per genotype. There was a significant decrease in the number of cells expressing claudin-2 in the mutant mice. P = .043 for NKCC1–DFX and P = .019 for KO. There was no difference between NKCC1-DFX and NKCC1 KO mice (P = .93). (G) Data are shown as whisker boxes and statistical significance was calculated by 1-way analysis of variance followed by Tukey posttests. CLDN2, claudin-2.