Figure 2.

Abnormal water and intestinal transit are observed in NKCC1-DFX mutant mice. (A) Water content of fecal pellets collected from WT NKCC1^WT/WT, NKCC1^WT/DFX, and NKCC1^DFX/DFX mice. Whisker plot data presents the median, lower and upper quartiles, and minimum and maximum for 9–12 pellets from 3–4 animals per group. (B) Gastrointestinal transit time measured by subtracting time of oral gavage with 6% carmine red solution from time of appearance of red fecal pellets (n = 3–4 mice per group). (C and D) The number of pellets increases in the colon of NKCC1^DFX/DFX mice. (A, B, and D) P values were obtained from 1-way analysis of variance with follow-up Tukey posttests.