Figure 6.

Loss of NKCC1 function impairs the organization of the outer and inner mucus layers. (A–C) UEA-1 lectin staining of colon section from (A and D) WT, (B and E) heterozygous, and (C and F) homozygous NKCC1-DFX mice. The inner mucus layer is thinner in the NKCC1^WT/DFX and NKCC1^DFX/DFX mice. Scale bars: 20 μm. (D–F) High magnification from indicated regions of panels A–C, respectively. (G) Quantification of the inner mucus layer thickness in the distal colon (n = 3 mice per group, 8 micrographs per mouse section). Data are shown as whisker boxes and statistical significance was calculated by 1-way analysis of variance followed by Tukey posttests. ***P < .001.