Figure 8.

Loss of NKCC1 function increases CD3+ lymphocytes infiltration in the colon. (A–C) Representative H&E-stained micrographs of colon sections from 8-week-old NKCC1-WT, NKCC1-DFX, and NKCC1-KO mouse colon sections showing overall normal anatomy. (D–G) Sections of same genotypes stained with anti-CD3 antibody. Scale bars: 20 μm. (G) Quantification of the number of infiltrating CD3+ cells per genotype (N = 3 mice per group; 8 micrographs per section were taken and counted). Data are shown as whisker box plots, and statistical significance was calculated by 1-way analysis of variance followed by Tukey posttests. *P < .05, **P < .01.