Value of the Data •These data on the effect of oral DBP exposure on uterine DNA methyltransferase activity provide insight into the mechanisms of action of this endocrine-disrupting chemical. •These data will benefit the fields of reproductive biology and toxicology by enhancing understanding of how environmentally-induced epigenetic alterations can affect normal uterine function, reproduction, and the development of several complex diseases that compromise women's health. •These data highlight the potential negative effects of phthalates on epigenetic regulation of uterine function; thus, they will stimulate the formulation of novel hypotheses and experiments aimed at elucidating the role of phthalate exposures on the development of epigenetically-driven uterine diseases.