Table 1.
Mono-iodoacetate (MIA) rat studies: Tabulated summary.
| Study | Rat strain | Body weight (g) (age) | MIA injected |
Observations* | ||
|---|---|---|---|---|---|---|
| Volume (µL) | Dose level (mg) | Dose (mg/kg) | ||||
| Guingamp10, 11 | Wistar | 150–225 | 50 | 0.3 | 1.6 | Day 1–2: Activity reduced Day 3–6: Normal activity Day 7+: Activity reduced |
| 50 | 3 | 16 | Rapid degradation | |||
| Janusz et al12 | Sprague-Dawley | 220–230 | 25 | 0.25 | 1.1 | Day 1–7: Progressive GAG loss Day 21: Subchondral degradation |
| Guzman et al13 | Wistar | 175–200 | 50 | 1 | 5.3 | Day 1: Chondrocytes degenerate Day 5–7: Cartilage and loss of chondrocytes Day 7–14: Subchondral bone marrow loss Day 28: Multiple collapse |
| Saito14 | Wistar | (10–11 wk) | 50 | 0.1 | 0.4 | Weak damage by MIA Degradation, 15 km walk |
| Peng et al15 | Lewis | (old males) | 50 | 1 | 4 | Day 3: Onset of pain, Day 14-28: Femorotibial and femoropatellar damages |
| Ferland-Legault16, 17 | Sprague-Dawley | 180–225 | 30 | 3 | 15 | Day 3, 7, 14, 21, 28: Structural damages Day 7-21: Similar to OA in human |
| Liu et al18 | Sprague-Dawley | 275–300 | 60 | 4.8 | 16 | High doses have been deliberately selected |
| Mannelli et al19 | Sprague-Dawley | 200–250 | 25 | 2 | 8.9 |
Day 0: Anti-O2− treatment Day 14: Slowed OA Day 14: MIA controls severely affected |
| Benschop et al20 | Wistar | 8 (wk) | 50 | 0.3 | 1.5 | NSAID responders : Day 14: Treatment Day 17: Pain evaluation |
| 50 | 1 | 5 | NSAID non-responders : Day 40: Treatment Day 43: Treatment and pain evaluation at +2 h |
|||
| Naveen et al21 | Sprague-Dawley | 100–150 (12 wk) | 25 | 2 | 16 | Cartilage thickness and GAG Day 7: Moderate degradation Day 14: Major degradation |
| Calado et al22 | Wistar | 250 | 25 | 2 | 8 | Day 3: Major handicap Study limited to 10 days |
| Jain et al23 | Charles Foster | 180–220 | 40 | 2 | 10 |
Day 7–14: Oral diacerein Day 28: OA slowed Day 56: Cartilage healed MIA controls: Day 7: Loss of hyalin cartilage Day 14: Deep fissures Day 28: Subchondral bone collapsed Day 56: Total loss of cartilage |
GAG = glycosaminoglycan; O2− = Superoxyde anion; OA = osteoarthritis; NSAID = non-steroidal anti-inflammatory drug
Observations are in italic characters for the groups receiving a treatment
⁎
MIA was injected on Day 0.