Figure 4.

Gene Organization of SPβ-Related Phages Inserted into the kamA Gene

(A) Synteny of SPβ-related phages residing in kamA; the top diagram shows the positions of the integrase (int) and putative recombination directionality factor (rdf) genes. Host strains and phage names are indicated on the left of the diagram and lengths on the right. Integrase amino acid sequence homologies (%) to Int_φ12-5 or SprA (Int_SPβ) are shown on the right column.

(B) Comparisons between the SPβ and φ3T phage genomes. The enlarged views on the left-hand side show the genes flanking the rdf. Vertical black arrowheads indicate the position of the integration site.

(C) Synteny of the SPβ, φ3T, and φ12-5 genomes. Genome data were extracted from the genome database at the national center for biotechnology information (NCBI). Sequence comparisons were performed using tBLASTx; red-blue lines indicate regions with 60%–100% identity. Genome alignment figures were created using Easyfig (Easyfig 2.2.2 for generating tBLASTx alignment files and visualization). Genetic homology (tBLASTx) percentages are included on the right of the diagram.

See also Figures S4 and S5 and Table S2.