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. 2019 Dec 28;23(1):100809. doi: 10.1016/j.isci.2019.100809

Figure 2.

Figure 2

Cytokinesis Defect of tdSMN Mutant Cells

(A) Comparison of LifeAct-GFP-labeled F-actin structures in wild-type, tdSMN, tdSMN acp1Δ suppressor, and acp1Δ cells. Fluorescence micrographs show that the S. pombe actin networks are observed in all four strains. The bar represents 3 μm.

(B) Quantification of (A) showing the percentage of cells with corresponding actin distribution for each indicated strain. Monopolar, actin localized at one end; bipolar, actin at both ends; cytokinesis, actin localized at the cytokinetic ring. Data are represented as the mean from three separate experiments (n = 100) with error bars showing the SD.

(C) Quantification of (A) showing the percentage of cells with corresponding cell sizes (in μm) for cells having actin localized as monopolar, bipolar, or at the cytokinetic ring. Results are from three independent measurements (n = 100). Quantification data are also shown as histograms in Figure S5.