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. 2019 Dec 30;23(1):100813. doi: 10.1016/j.isci.2019.100813

Figure 1.

Figure 1

WT Embryonic Mouse Brain Supports the Engraftment and Growth of a Human GBM Cell Line (TX)

(A) Experimental procedure: (I) Preparation of single cell suspension of dsRed-labeled tumor cells, (II) surgery, and (III–V) tumor cell injection in the lateral ventricle (LV) of E12.5 mouse embryos; Cx, cortex; LGE/MGE, lateral/medial ganglionic eminence.

(B) Dorsal view at E18.5 and P7 mouse brains with TX; white arrowheads: dsRed+ tumors; red arrowheads: tumors in higher magnification (insets); OB, olfactory bulb; dT, dorsal telencephalon; M, midbrain; H, hindbrain; dashed lines indicate brain compartment boundaries. Scale bar: 2 mm; insets: 500 μm.

(C) Number of tumors per brain at the indicated time points, n = number of brains with tumors; light gray: N°/single brains, red: mean N°/age.

(D) Immunofluorescence images of cryosections through mouse TX at indicated ages; blue: Nuclei (Hoechst+), red: dsRed+ U87MG cells, and green: HuNu; T, tumor; H, host tissue; *, ventricle (same nomenclature in all the figures); scale bar: 200 μm.

(E) Tumor volume at the indicated time points [log10 scale]; light gray: single tumors, red: mean volume/age, n = number of brains with tumors.

(F) Nuclear density at E18.5 (n = 3) and P7 (n = 3).

(G) Number of total Hoechst + nuclei within TX per brain at E13.5 (n = 7 tumors in 4 brains), E15.5 (n = 4 tumors in 3 brains), and E18.5 (n = 6 tumors in 4 brains).

(H) Proportion of dsRed+ or HuNu + cells at E13.5 (n = 4), E15.5 (n = 3), and E18.5 (n = 4) over total Hoechst + nuclei in TX.

Data are represented as mean ± SEM; statistical significance revealed by using one-way ANOVA and Tukey's multiple comparisons test. * p value < 0.05; ** p value < 0.01; *** p value < 0.001; **** p value < 0.0001; n.s., not significant.