Figure 2.

WT Embryonic Mouse Brain Supports Growth of Patient-Derived GBM Xenografts (PDX)

(A) Left image: dorsal view at E18.5 embryonic mouse brain with PDX; arrowheads: dsRed+ tumors; dashed lines indicate brain compartment boundaries. Center and right: 3D reconstruction of mouse brains at the indicated age showing PDX in red. Note the dramatic increase in tumor volume between the two time points. Scale bar: 1 mm.

(B) Graphs showing (from left to right) Number of tumors per brain (brains n = 3); tumor volume [log10 scale] (tumors n = 10 in three different brains per condition), and percentage of brain volume occupied by tumors (brains n = 3), at the indicated time points.

(C) Immunofluorescence images of PDX in coronal cryosections through embryonic mouse brains at E18.5 and P7; blue: Nuclei (Hoechst+) and graphical representation of nuclear density in PDX tumors at the indicated ages (tumors n = 7 in three different brains per condition).

(D) Immunofluorescence images of PDX in coronal cryosections through embryonic mouse brains at E18.5; blue: Nuclei (Hoechst+), red: dsRed+ patient-derived cells, green: HuNu, cyan: KI67; white: GFAP (center panel) or S100β (bottom panel).

(E) Graphical representation of (top) Total number of Hoechst + nuclei within PDX at E18.5 per brain (n = 12 tumors in 5 brains); (center) proportion of dsRed+ or HuNu + cells at E18.5 (n = 5) over total nuclei in PDX; (bottom) proportion of KI67 + +dsRed+ cells over total dsRed+ cells in PDX at E18.5 (n = 3).

Data are represented as mean ± SEM; statistical significance revealed by using unpaired Student's t test. * p value < 0.05; ** p value < 0.01; n.s., not significant.