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. 2019 Dec 30;23(1):100813. doi: 10.1016/j.isci.2019.100813

Figure 4.

Figure 4

TX and PDX Develop Functional Vasculature in Brains of WT Mice

(A and B) Immunofluorescence images of TX (left panels) and PDX (right panels) in cryosections of embryonic mouse brains at E18.5 (blue: Nuclei (Hoechst+), red: dsRed+ cells) showing (A) white: immune reactivity for VEGF-A; (B) white: immune reactivity for the endothelial marker CD31 inside TX (left panels) and PDX (right panels).

(C–E) (C) Experimental procedure of FITC-Dextran injection, (D and E) Immunofluorescence images of coronal cryosections through embryonic brains at E18.5 showing green: 4 kDA FITC-Dextran, red: dsRed+ U87MG (TX) or Patient-derived GBM (PDX) cells; (D) low magnification image of FITC-Dextran labeled blood vessels in embryonic mouse brain and tumor (T); (E) high magnification of inset in (D) (left panels) and similar example on a vascularized tumor formed by patient-derived GBM cells (right panels) showing co-localization (arrows) of FITC-Dextran with dsRed + tumor cells. Scale bars: 100 μm in (A) and (B), 500 μm in (D), and 50 μm in (E).