Figure 5.

Embryonic Engraftment of Human GBM Cells in WT Mice Allows Investigation of Tumor Interaction with the Host Immune System

(A) Lymphocytes in bone marrow (BM) of WT control mice at P7 (n = 2), P15 (n = 2), and P21 (n = 6) relative to BM of adult (20–29 weeks) WT females (n = 4; red line).

(B) Lymphocytes in BM of P21 mice (TX n = 5 and PDX n = 11), normalized for CTL mice (n = 4).

(C) Lymphocytes in peripheral blood of P21 mice (TX n = 5 and PDX n = 14) normalized for CTL (n = 6).

(D) Ventral view at P21 and P28 mouse brains with TX or PDX; arrowheads: dsRed+ tumors; OB, olfactory bulb; Cx, cortex; Hy, hypothalamus; dashed lines: brain compartment boundaries. Scale bar: 2 mm.

(E) Number of tumor-positive brains as % of total brains dissected at P7 (TX n = 10, PDX n = 7), P15 (n = 23; n = 8), P21 (n = 24; n = 25), and P28 (n = 29; n = 19).

(F) Cell composition in tumors at P7 and P28 in TX (n = 3; n = 1) or PDX (n = 4; n = 2) (proportion of dsRed+ [red] or IBA1+ [cyan] or Hoechst + dsRed-IBA1- [blue]) over total Nuclei/field.

(G) Immunofluorescence images of TX in cryosections through E18.5 brains as example of activated IBA1+CD68+ (G’’, arrow) and non-activated IBA1+CD68- (G’); blue: Nuclei (Hoechst+), red: dsRed+ U87MG cells, cyan: IBA1, and white: CD68; scale bar: 20 μm.

(H) Proportion of IBA1+ cells at E18.5, P7, P21, and P28 in TX (n = 3, n = 3, n = 1, n = 1) or PDX (n = 5, n = 4, n = 6, n = 2) over total nuclei/field.

(I) Proportion of activated IBA1+CD68 + cells at E18.5, P7, P21, and P28 in TX or PDX over total IBA1+ cells/field (value of n as presented in [H]).

Data are represented as mean ± SEM; statistical significance revealed by using unpaired Student's t test and one-way ANOVA followed by Tukey's multiple comparison test. * p value < 0.05; n.s., not significant.