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. 2020 Jan 8;13:173–183. doi: 10.2147/OTT.S240113

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© 2020 Zhang et al.

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LINC00473 targeted miR-345-5p. (A) The binding sites of LINC00473 and miR-345-5p were shown. (B, C) Luciferase activity was detected in Huh-7 and SK-HEP-1 cells co-transfected with LINC00473-WT or LINC00473-MUT and miR-345-5p or miR-NC by dual-luciferase reporter assay. (D) The level of miR-345-5p was determined in HCC tissues and adjacent normal tissues. (E) Spearman correlation analysis was used to analyze the correlation between LINC00473 and miR-345-5p levels in HCC tissues. (F) The expression of miR-345-5p was examined in THLE-2 cells and HCC cell lines (Huh-7 and SK-HEP-1). (G, H) The level of miR-345-5p was detected in Huh-7 and SK-HEP-1 cells transfected with Vector, LINC00473, si-NC or si-LINC00473#1. *P < 0.05.