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. 2018 Jan 1;11(1):314–323.

Figure 2.

Figure 2

The overexpression of PlncRNA-1 increases proliferation and inhibits apoptosis and autophagy in LPS-induced septic AKI cells. NRK-52E cells were treated with LPS to establish the septic AKI model. LPS-induced septic AKI cells were transfected with pc-PlncRNA-1 and blank. A. MTT assay showing cell viability of different treated groups. B. Colony formation assay showing the colony number in different treated groups. C. Flow cytometry showing cell apoptosis in the different treated groups. D. Western blot showing the expression levels of apoptosis-related proteins (active caspase-3 and -9) in the different transfected groups. E. Immunofluorescence assay showing the positive cells with LC3 puncta in the different transfected groups. F. Western blot showing the expression levels of autophagy-related proteins (LC3-II/I ratio and Beclin-1) in the different transfected groups. *P < 0.05, **P < 0.01, ***P < 0.001 compared with corresponding controls.